DIFFUSION PLATE ASSAY FOR CHLORAMPHENICOL AND AUREOMYCIN 1
نویسندگان
چکیده
منابع مشابه
Dual-substrate plate diffusion assay for proteases.
A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a range from 0.01 to greater than 100 IU/ml.
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An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system. Serum levels of chloramphenicol as low as 2.0 mug/ml could be reproducibly determined with thi...
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We partly purified R-factor-encoded chloramphenicol acetyltransferase (EC 2.3.1.28) from a highly choloramphenicol-resistant mutant derived from Escherichia coli W677/R5. The preparation permitted rapid quanitation of chloramphenicol by use of [14C]acetylcoenzyme A, removing the diacetylated product by selective adsorption onto micropore filters. Succinyl and glucuronyl 3-hydroxyl esters of chl...
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In a previous paper (Tomaszewski, 1951) a survey was made of the side-effects of chloramphenicol and aureomycin treatment. These were divided into general, gastro-intestinal, genito-rectal, and oral manifestations. Changes in the oral cavity were found more frequently than in other parts of the body. They consisted of dryness of the mouth, throat manifestations, angular stomatitis, and changes ...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1949
ISSN: 0021-9738
DOI: 10.1172/jci102183